Three urine/fluid samples were taken from each of the two female subjects during our second trial run on June 22, 1996 (see Table I).  A baseline urine specimen was obtained before the stimulation began and both specimens showed relatively normal urea and creatinine counts.

Table I
Laboratory Results for Experiment of June 22, 1996

		Subject 1	Subject 2
Baseline Urine Specimen	Urea	240 mg/dl	290 mg/dl
	Creatinine	32 mg/dl	42 mg/dl
Catheter bag with "drained" bladder contents (after 1 hour of sexual stimulation)	Urea	80 mg/dl	190 mg/dl
	Creatinine	8 mg/dl	27 mg/dl
catheter bag with "unknown" fluid (after additional sexual stimulation	Urea	80 mg/dl	140 mg/dl
and percieved ejaculatory orgasm)	Creatinine	10 mg/dl	18 mg/dl
Blood Tests
PSA resting		< 0.20 ng/ml	< 0.20 ng/ml
PSA after arousal and orgasm		< 0.20 ng/ml	< 0.20 ng/ml
Perineometer Reading
		80.00 mm/Hg	75.00 mm/Hg

_____________________________________________________
Laboratory results by SmithKline Beecham Clinical Laboratories

The female subjects then began an arousal period of at least an hour in whatever manner was preferable to them before the actual insertion of the Foley catheter.  The choices utilized were manual self stimulation, manual stimulation by a partner, and/or use of a non mechanical acrylic device known as a Crystal or G Spot wand.  After the initial period of  sexual arousal and when the subjects indicated that they were ready, the catheter was inserted, their bladders were drained and the catheter bags were changed.

The contents of the bags containing urine/fluid from the drained sample showed a substantial decrease in urea and creatinine levels, particularly in Subject #1.  The rate of decrease for the contents of the bag for Subject #1 was 67% for urea and 75% for creatinine.  For Subject #2, the rate of decrease was 35% for urea and 36% for creatinine.

After their bladders were drained, the women continued to be stimulated in the aforementioned manners. Over a period of an hour and a half they expelled a clear fluid in pulses, roughly coinciding with their orgasmic contractions, into the new catheter bags.  The volume of fluid was approximately 540 ml for Subject #1 and 330 ml for Subject #2.  Analysis of the urine/fluid from the last catheter bags showed virtually the same urea and creatinine levels for Subject #1 and an additional drop of 17% urea and 21% creatinine for Subject #2.  Therefore, the total rate of decrease from the baseline urine specimen to the post draining urine/fluid captured in the last catheter bag for Subject #1 was 67% for urea and 75% for creatinine.  For Subject #2, the total rate of decrease was 52% for urea and 57% for creatinine.

A glucose test was run on the urine/fluid samples from both subjects from June 22, 1996 and, while some earlier studies showed a rise in glucose from the baseline urine sample to the expelled fluid, in these subjects the glucose level remained relatively constant.

In the case of Subject #2 on June 22, 1996, there was a point in time in which there was an unmistakable expulsion of approximately two cc of milky-white fluid through the urethral meatus and completely surrounding the outside of the catheter tube.  Unfortunately, the research team was unable to capture a sample of this fluid for analysis and the lighting for the videotape was insufficient to show the expulsion.

On Saturday, July 27, 1996, six female subjects were tested at a facility in Tiburon, California.  Subject #1 was a retest of Subject #2 from June 22, 1996. A baseline urine sample was taken and a perineometer test was performed on the female subjects upon their arrival at approximately noon (see Tables II and III).  During the next two hours an orientation took place as to the procedures to be followed.  Questions were answered and concerns were addressed.

At approximately 2:00 PM, an arousal period was begun for the female subjects with a variety of methods available to achieve that end.  The choices that were utilized were manual self stimulation, manual stimulation by a partner, and/or use of a non mechanical acrylic device known as a Crystal or G Spot wand.  When the subjects felt that they were sufficiently aroused and indicated that they were ready to begin the experiment, they were ushered into the first available examination room.  Arousal was then resumed in the aforementioned manners until the female subjects indicated that they were ready to have the catheter inserted and their bladders drained.  Stimulation was then resumed with the subjects instructed to attempt to achieve an expulsion of fluid in a manner similar to their normal patterns.

In all six cases, the female subjects expelled substantial amounts of fluid from their bladders, post draining, in pulses roughly coinciding with their contractions, through the catheter tube and into the storage bag.  These amounts ranged in volume from approximately 50 ml for Subject #5 to 900 ml for Subject #1.  In all cases, the urea and creatinine levels of the fluid expelled after the draining of the bladder were substantially lower than the levels for the same components in the baseline urine specimen by an average of 72% for urea and 76% for creatinine.  In a number of cases, the urea and creatinine levels decreased substantially just between the baseline and drained specimens.  In the case of Subject #1, after an hour of stimulation, the catheter tube and inflated balloon were expelled.  A new catheter tube was inserted and a new post-draining bag was started. Laboratory results are in Table II.

Table II
Specimens from July 27, 1996 Testing

Subject		Baseline Urine A	Drained Urine B	Post-drain Cath. C	Post-drain Cath. D	Urethral Syringe D
1	Amount		75 ml	500 ml	400 ml
	Urea	162	205	102	96
	Creatinine	19	36	15	16
	Fructose			Neg.	Neg.
2	Amount		10 ml	100 ml
	Urea	541	435	398
	Creatinine	76	46	46
	Fructose			Neg.
3	Amount		125 ml	500 ml
	Urea	605	91	87
	Creatinine	120	10	9
	Fructose			Neg.
5	Amount		75 ml	50 ml
	Urea	892	148	144
	Creatinine	144	20	19
	Fructose			Neg.
7	Amount		250 ml	100 ml
	Urea	209	214	138
	Creatinine	25	26	19
	Fructose			Neg.
8	Amount		50 ml	100 ml		1 cc
	Urea	903	126	130		110
	Creatinine	106	12	12		8
	Fructose			Neg.		Neg.

_________________________________________________
Urea and Creatinine in mg.dl
Laboratory results by LabOne, Overland Park, Kansas
 

As for any fluid that came from the urethral opening but outside of the catheter tube, attempts were made by the medical team to capture the fluid by drawing it into a 10 cc syringe or catching it with a laboratory specimen container.  The research team saw clear visual evidence, recorded by the videotape, of milky-white, mucous-like emissions from the urethra outside of the catheter tube for subjects #1, 3 and 8.

Trace amounts of fluid from outside the catheter tube were captured from subjects #1, 2 and 3 but were insufficient in volume for the laboratory to test.  The samples were frozen and are being stored should the opportunity become available to test these tiny amounts.  In a fourth instance, Subject #8 produced approximately 1 cc of a clear, thick liquid that tested negative for fructose and glucose.  Urea and creatinine levels were slightly lower but similar to those of the same subject’s fluid expelled into the catheter bag subsequent to her bladder being drained.  It is possible that the 1 cc of fluid in question represented leakage from the point where the bladder neck met the catheter balloon.

In the case of subject #3, the research team completely overlooked an apparent emission at the end of the testing session that is clearly shown by two of the three video cameras.  No comment was made at the time nor was any movement made towards collection of a sample of that emission.  In reviewing the videotape, it would appear that a number of factors contributed to our not being aware of what was taking place.  First, the subject was very quiet and gave no verbal indication of an impending orgasm.  Secondly, the subject’s husband was wearing a white latex glove while gently tapping on her clitoris at the time of orgasm.  He left his finger in a down position on her clitoris for a few seconds after the orgasm and it is not until he raises his finger that the viewer is able to see the milky-white, mucous-like fluid.  In real time, it was probably missed because of the lack of color contrast with the glove.  Also, the video cameras, with their zoom lenses, amplified the emission, making the event easier to see.

In five of the seven subjects (Subjects #2, 3, 7, 8 from 7/27/96 and Subject #1 from 6/22/96), the research team observed the phenomenon referred to as eversion.⁵⁹   This occurs when the urethral meatus appears to push outward and becomes very visible just prior to orgasm.  In Addiego et al, it was stated that this occurred when the Gräfenberg spot was stimulated.  It should be noted that Subject #3 at no time utilized G spot stimulation and still exhibited signs of eversion.  Also, Subject #7 exhibited eversion with only clitoral and anal stimulation and then with clitoral, Gräfenberg spot and anal stimulation.

Table III
Perineometer Readings from July 27, 1996 Testing

Subject	Time In	Relax	Contract	Muscle  Strength
1	12:13 pm	28	40	12
2	12:32 pm	14	42	28
3	12:28 pm	20	42	22
5	12:22 pm	16	40	24
7	12:07 pm	20	62	42
8	12:16 pm	24	56	32

_____________________________________________________
Muscle strength was registered on a Perineometer as the change in pressure measured in millimeters of mercury (mm/Hg) between the average of six 10-second contract scores, minus the average of six 10-second relax scores.

59. Addiego et al, pg. 16

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