The experiment was constructed for the current study to take a urine specimen from the female subjects while in a resting state.  The initial urine was analyzed for 2 factors, urea and creatinine.

The female subjects then began an arousal period of at least an hour in whatever manner was preferable to them before the actual insertion of the Foley catheter.  The choices that were utilized were manual self stimulation, manual stimulation by a partner, and/or use of a non-mechanical acrylic device known as a Crystal or G Spot wand.

After the subjects indicated that they felt properly stimulated and ready,  the catheter was inserted and the balloon filled so as to form a seal with the bladder neck.  Their bladders were drained and the collection bag was changed.  The bag with the drained fluid was saved for analyses for the same 2 factors, urea and creatinine.

Then, with the catheter in place, the subjects were asked to resume stimulation and achieve what they considered to be an ejaculatory orgasm in whatever manner was preferable to them except intercourse, due to the catheter.  The choice of stimulation employed were again manual self stimulation, manual stimulation by a partner, and/or use of the G Spot wand.

Any expulsion of fluid through the catheter tube went into the new bag for analysis for the same 2 factors, urea and creatinine.  If any fluid was expelled outside the tube and bag, an attempt was made to collect it in a specimen container and it was analyzed for fructose and glucose.  If there had been any  indication of these prostatic components, then tests would have been done for the presence of Prostate Specific Antigens.¹

A perineometer test was administered before the arousal period began to measure the pelvic muscle strength of our subjects.  The purpose was to compare the readings with those from research on urinary stress incontinence and previous perineometer testing done by Arnold Kegel, M. D. among others.²

The entire experiment was videotaped with a medical doctor and/or a registered nurse present at all times.  The overall environment was designed to be as comfortable and natural as possible to increase the probability that there would be fluid to collect.

A sex history was taken on all subjects along with questions pertaining to the phenomenon being studied (see Appendix A).  Examples of such questions included, but were not limited to, when the female subjects began to experience a discharge of fluid, whether they had done anything to train themselves to facilitate a discharge, and how they feel about the discharge.  The questionnaire was also designed to ask many of the same questions that were asked in a number of previous studies.³

In addition, a medical history was taken on each subject and information was distributed about the possibility of bladder or urethral irritation or infection from the experiment, with a procedure set up for follow-up treatment should any symptoms appear.  A full signed consent form was obtained.

With catheterization, the bladder could be isolated from the urethra so that it could be reliably determined which fluids came from which area.  The fluids obtained could then be analyzed for their individual composition while lessening the possibility of mixture in the urethra.  One concern about the catheter that was expressed and considered was the idea that the catheter tube might physically block an expulsion of fluid from the urethral glands and ducts.  That seemed to be a reasonable concern, so the research team experimented with ever smaller sizes of catheter tubes, starting out with a 12 FR in the initial trial and progressing to an 8 FR for the final testing session.  While it is still possible that the urethral glands and ducts were blocked, the research team was reluctant to discard the use of the Foley catheter as it appeared to be the best way to isolate the bladder from the urethra.

In two initial trials, procedures were checked and validated in terms of being able to produce an expulsion while the catheter was in place.  The final testing date occurred on July 27, 1996, when six of the seven subjects were tested.

1. Graves, pg. 338-343
2. Kegel, 1956, pg. 497
3. Perry and Whipple, 1981; Davidson, et al, Survey Instrument

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